Coding
HlyD

Part:BBa_K4387998:Design

Designed by: Nathalie Weibel   Group: iGEM22_UZurich   (2022-09-28)


HlyD of the hemolysin A one-step secretion system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence originally contained a SapI restrictions site which was removed by performing a silent mutation. HlyD is found in the composite part BBa_K4387987, allowing the constitutive expression of the HlyA secretion machinery.


Source

Sequence of the HlyD was obtain from the paper of Ruano-Gallego et al. [1]


References

[1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0